Fig. 4

Growth hormone increases the expression of MMP-2 in B16F10 cells through JAK signaling. a Expression of MMP2 mRNA was increased in B16F10 cells following 3 h of treatment with GH in a concentration-dependent manner. b MMP-2 expression was increased by 10 ng/ml in a time-dependent manner. c B16F10 cells treated with GH (0.1, 1, 10 ng/ml) for 6 h increased expression of MMP-2 protein in a concentration-dependent manner. d GH-induced increase of MMP-2 was inhibited by JAK inhibitor (AG490). Data are presented as mean ± SEM (n = 3 for each group); *, P < 0.05 compared to the control (Con); #, P < 0.05 compared to GH treatment alone; JAK, Janus kinase