Fig. 3

Growth hormone enhances colony formation and invasive capacity of B16F10 melanoma cells. a Colony formation of B16F10 cells in soft agar with and without GH. Note that GH increased B16F10 cell colony formation in a concentration-dependent manner and colonies were photographed and counted. b B16F10 cells were seeded into a transwell with 8-μm pore polycarbonate filters and matrix gel. Cells penetrated the pores to the lower surface of filters and were stained with crystal violet and counted. The results showed that GH increased the invasive capacity of B16F10 cells in a concentration-dependent manner. Data are presented as mean ± SEM (n = 4 for each group); *, P < 0.05 compared to the control. Scale bar = 0.2 mm