Fig. 1
Impaired murine tumor cell proliferation after treatment with Salinomycin. 5 × 103 MC38 (a + b) and CT26 (c + d) cells were cultured in microtitre plates in the absence or presence of 1 μM 5-FU, increasing concentrations of Salinomycin (1 μM, 2 μM, 5 μM and 10 μM) and a combination of 5-FU and Salinomycin. Treatment was performed either for 24 h and 48 h or for another 24–48 h with fresh medium. Tumor cell proliferation was performed using the WST-1 assay. Results are displayed as summary of at least 3 independent experiments as mean ± SD; *p < 0.05 and **p < 0.001 compared with control