Fig. 1
NH125 sensitizes NPC cells to lapatinib. a, b and c NPC cells were treated with lapatinib or DMSO for 48 h in the presence or absence of 0.25 μM NH125. a Cell viability was assessed by the CCK-8 assay. Results are expressed as means ± standard deviation. *, P<0.05;**, P<0.01 and ***, P<0.001. b Inhibition of proliferation was measured by the crystal violet assay. HONE-1 cells are shown in a representative experiment. c Colony formation was measured. CNE-2 cells are shown in a representative experiment d and e CNE-2 and HONE-1 cells were treated with lapatinib or DMSO under normal or hypoxic (1 % O2) conditions for 48 h. d HIF-1α and phosphorylated eEF-2 levels were examined by Western blot analysis. GAPDH was used as a loading control. e Cell viability was assessed by the CCK-8 assay