Fig. 1

Blockade of N-cadherin cleavage decreased m ϕ CM-induced cell invasion and MMP-9 levels. a m ϕ CM enhanced NPC cell invasion. NPC cells were seeded into the inner well of the Boyden chamber, pre-coated with matrix-gel and monoCM or m ϕ CM, then introduced into the outer well of the Boyden chamber for 24 h. Cells that invaded the lower surface of the filter were fixed, stained, photographed and counted. Representative plots of Matrigel invasion assay are shown. Data are mean±SEM. N = 3, ^*P<0.05. b NPC cells were treated with monoCM or m ϕ CM for 24 h, then cell lysates underwent western blot analysis with indicated antibodies (N-cad, E-cad and MMP-9). c Blockade of N-cadherin cleavage by γI decreased m ϕ CM-induced MMP-9 expression in NPC-TW039 cells. NPC cells were pre-treated with γI (5 μM), then exposed to m ϕ CM for 24 h. Cell lysates underwent western blot analysis with indicated antibodies