MiR-9 is overexpressed in spontaneous canine osteosarcoma and promotes a metastatic phenotype including invasion and migration in osteoblasts and osteosarcoma cell lines

Table 3 Proteins identified by two-dimensional electrophoresis and capillary-liquid chromatography-tandem mass spectrometry

Spot #	t-test	Av Δ	Acc #	Description	pI	Mass	S^a	M^b
291	0.038	−1.4	gi\|73997540	PREDICTED: ELKS/Rab6-interacting/CAST family member 1 isoformX1 [Canis lupus familiaris]	5.69	128164	147	2
919	0.007	−1.1	gi\|73980394	PREDICTED: protein disulfide-isomerase A6 [Canis lupus familiaris]	4.97	48667	906	11
961	0.021	−1.3	No ID
1080	0.034	1.1	No ID
1384	0.033	1.2	No ID
1609	0.027	1.4	gi\|5031635	cofilin-1 [Canis lupus familiaris]	8.52	19715	343	3
1617	0.03	1.2	No ID
1872	0.029	1.2	gi\|478533920	PREDICTED: 60S acidic ribosomal protein P2 [Ceratotherium simum simum]	4.44	11772	364	4
1925	0.052	1.2	gi\|350582724	PREDICTED: 14-3-3 protein theta isoform 2 [Sus scrofa]	4.67	28075	983	12
1935	0.008	1.2	gi\|545518174	PREDICTED: gelsolin [Canis lupus familiaris]	8.49	95129	609	8

^aThe protein score is derived from Mascot and provides an indication of how well the peptides matched the indicated protein sequence. The actual score is calculated by the following equation: protein score = −10*Log(P), where P is the probability that the protein match is a random event. Scores above 100 indicate that p < 0.05
^bThe protein match score indicates the number of unique peptides that matched the sequence of the identified protein. Two unique peptide matches to a protein sequence confirms the identity of a prot

ISSN: 1471-2407