Fig. 6

MiR-9 alters gelsolin expression in normal canine osteoblasts and OS cell lines. a Protein lysates were generated from normal canine osteoblasts stably transduced with either empty vector (EV) or pre-miR-9-3 (miR-9) lentivirus and canine OSA8 cells stably transduced with miRZip-9 (anti-miR-9) or scramble vector control. Protein lysates were separated via SDS-PAGE and western blotting for gelsolin and β-actin was performed. b Canine osteoblasts expressing pre-miR-9-3 lentivirus or empty vector control were collected and real-time PCR for gelsolin and (c) TGFBI was performed (Bars: SD. Statistical analysis: one-way ANOVA, *p < 0.01). Three independent experiments were performed using cells from 3 separate transduction experiments and all reactions were performed in triplicate