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Fig. 3 | BMC Cancer

Fig. 3

From: MiR-9 is overexpressed in spontaneous canine osteosarcoma and promotes a metastatic phenotype including invasion and migration in osteoblasts and osteosarcoma cell lines

Fig. 3

MiR-9 is highly expressed in primary canine OS tumors and canine OS cell lines. RNA was collected from primary canine osteoblast cultures (CBDC, n = 5), normal canine osteoblast cell lines (Ob, n = 2, corresponding to 2 different commercially available lots), normal canine bone (n = 5), canine OS cell lines (n = 8), FFPE canine OS tumor cores (n = 5), and fresh primary canine OS tumors (n = 20) and real-time PCR was performed to evaluate miR-9 expression. Real time PCR confirmed overexpression of miR-9 in canine OS tumors and OS cell lines as compared to normal canine osteoblasts (p < 0.001). Three independent experiments were performed and all reactions were performed in triplicate

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BMC Cancer

ISSN: 1471-2407

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