Fig. 2
From: Aldehyde dehydrogenase activity plays a Key role in the aggressive phenotype of neuroblastoma
ALDH activity remains stable during NB TICs selection. ALDH activity was measured using the ALDEFLUOR kit in parental cells (T0) and successive sphere passages (s1 to s4) in SK-N-Be2c, NB1-C, and NB1 PDX-derived cells. a The percentage of ALDH+ cells (black bars) and the mean fluorescence intensity (MFI) ratio (MFI of the test tube/control tube, red bars) are plotted as mean ± SD of more than 3 experiments according to cell availability at each sphere passage (unpaired t-tests: ***p < 0.0005). b Representative dot plots showing the ALDH activity in SK-N-Be2c and NB1-C parental cells (T0) and in sphere passages 4 (s4) in presence (control tube) or absence (test tube) of DEAB. The percentage of ALDH+ cells are indicated in the dot plots