Fig. 7

The pan-HDACi TSA mediates its anti-proliferative effects on LECs through p21 dependent pathways. a Cells were treated with siRNA against p21, p53 (b) and control siRNA and were exposed to 400 nM TSA or solvent only (=Ethanol; 100 %) for 24 h as indicated. Cell proliferation was measured using the BrdU assay. Average absorbance values (mean ± SE) from 3 wells per experimental condition are displayed; data are expressed as cell proliferation in percentage (%) with regard to solvent controls (=100%; ethanol). *^,**p < 0.05 vs Ctrl. c Representative Western blot analyses of LECs that were incubated with siRNA against p53 or control siRNA and treated with TSA (at 400 nM) or solvent only (Ethanol) as indicated for 24 h. p53, p21 and α-Tubulin protein as loading control were detected by enhanced chemiluminescence. Comparable results were obtained from three independent experiments