Fig. 7
Plgf is expressed in neutrophils of HOXB7-Cre; Vhlhfl/fl knockout mice. a, b Plgf immunohistochemistry of liver sections (brown stain). Nuclei were stained with hematoxylin (light blue). Representatives Plgf + (a) and Plgf- (b) neutrophils localized within hemangiomas of a HOXB7-Cre; Vhlhfl/fl knockout mouse are shown. Note the polymorpho-nuclear shape characteristic of neutrophils. c Quantification of Plgf-expressing liver neutrophils (immunohistochemistry); n = 3 mice per group, total number of neutrophils counted: 38 (WT) and 510 (KO). Neutrophils were classified as strongly Plgf positive (Plgf+, dark pink), weakly Plgf positive (Plgf+/-, white) or Plgf negative (Plgf-, grey). A ~10-fold increase in the percentage of Plgf + neutrophils was observed in HOXB7-Cre; Vhlhfl/fl mice compared to Cre-negative littermates (46 % versus 4.4 %)