Fig. 3
Laser scanning confocal microscope images of fluorescently labelled live MCF-7CC10 and MCF-7DOX2–10 cells (a-d) or identical cell lines fixed and immunohistochemically-stained with epitope-specific antibodies (e-h). Panels A through D represent cells stained with MDC (blue) and LysoTracker® (green) with or without doxorubicin treatment. In panels E and F, cells were fixed in formaldehyde and immunohistochemically stained with anti-LC3-II (green) and anti LAMP1 (red) antibodies. For panels G and H, cells were labelled with MitoTracker™ (red) and followed by formaldehyde fixation and immunocytochemical staining with an anti-LC3-II antibody (green) after 24 h treatment with 10 μM chloroquine (CQ) prior to labelling. The images in this figure are representatives of approximately 100 cells examined on two separately stained slides. Each image represents one of 10 microscopic photos taken from two independent experiments. The staining and phenotype consistency were also confirmed in at least two biological replicates by 3D image/video (data not shown)