Fig. 3
Cell proliferation and migration of MBCDF’s subpopulations. a For cell proliferation, B3, B6, C1, D5 (Group 1), B2, F3, C9 (Group 2) subpopulations were seeded at 15 000 cells/cm2 in 24-well plate in RPMI plus 10 % FBS. Cell proliferation was evaluated by MTT at the days 0, 2, 4, and 6. Results are presented as the mean ± SEM of three independent experiments seeded in triplicate. ** P < 0.05. b Migration assays were performed using Boyden chambers. Thirty thousands cells of B3, B6, C1, D5 (Group 1), B2, C9, F3 (Group 2) subpopulations were seeded in the upper chamber in RPMI plus 10 % FBS and incubated for 6 h at 37 °C. After this time the cells that did not migrate were removed from the upper chamber. Cells that migrated were fixed and stained with Cristal Violet. Five fields were counted under the microscope at 20×. c Representative pictures of Boyden chamber assays. The percentage of migrations was calculated as mentioned in Materials and Methods. Migration assays was performed three independent times in triplicate. * P < 0.05