Skip to main content

Advertisement

Table 2 The change in the expression of the possibly linked genes in HepG2 cells and normal HepaRG® hepatocytes detected by microarray analyses (200 mg/L glucose vs 900 mg/L)

From: microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line

miRNA Glucose (mg/L) Ratio mRNA Glucose (mg/L) Ratio
900 200 900 200
a) HepaRG® (normal hepatocytes)
miR-15b-5p 51 25.1 0.49 HSPA1B 139 140 1.01
miR-16-5p 325.5 209.2 0.64 HSPA8 5386 5148 0.96
miR-17-5p 162.9 96.8 0.59 CDKN1A 1439 1457 1.01
miR-20a-5p 122.7 76.5 0.62     
b) HepG2
miR-15b-5p 243.3 148.8 0.61 HSPA1B 287 2009 7.00
miR-16-5p 1133.7 792.8 0.70 HSPA8 9925 11574 1.17
miR-17-5p 2144.4 1479.7 0.69 CDKN1A 291 713 2.45
miR-20a-5p 1793.2 1219.7 0.68     
  1. The expression of HSPA1B mRNA was seen to be regulated by miR-15b-5p and miR-16-5p, and this regulatory relationship was observed in the results of microarray analyses in HepG2 cells; HSPA1B mRNA was not increased in HepaRG® cells. HSPA8 transcription was not changed as much in either cell type. The expression of CDKN1A mRNA was seen to be regulated by miR-17-5p and miR-20a-5p, and this relationship was observed in HepG2 cells; CDKN1A mRNA transcription did not change in HepaRG® cells