microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line

BMC Cancer

Table 2 The change in the expression of the possibly linked genes in HepG2 cells and normal HepaRG® hepatocytes detected by microarray analyses (200 mg/L glucose vs 900 mg/L)

miRNA	Glucose (mg/L)		Ratio	mRNA	Glucose (mg/L)		Ratio
miRNA	900	200	Ratio	mRNA	900	200	Ratio
a) HepaRG® (normal hepatocytes)
miR-15b-5p	51	25.1	0.49	HSPA1B	139	140	1.01
miR-16-5p	325.5	209.2	0.64	HSPA8	5386	5148	0.96
miR-17-5p	162.9	96.8	0.59	CDKN1A	1439	1457	1.01
miR-20a-5p	122.7	76.5	0.62
b) HepG2
miR-15b-5p	243.3	148.8	0.61	HSPA1B	287	2009	7.00
miR-16-5p	1133.7	792.8	0.70	HSPA8	9925	11574	1.17
miR-17-5p	2144.4	1479.7	0.69	CDKN1A	291	713	2.45
miR-20a-5p	1793.2	1219.7	0.68

The expression of HSPA1B mRNA was seen to be regulated by miR-15b-5p and miR-16-5p, and this regulatory relationship was observed in the results of microarray analyses in HepG2 cells; HSPA1B mRNA was not increased in HepaRG® cells. HSPA8 transcription was not changed as much in either cell type. The expression of CDKN1A mRNA was seen to be regulated by miR-17-5p and miR-20a-5p, and this relationship was observed in HepG2 cells; CDKN1A mRNA transcription did not change in HepaRG® cells

ISSN: 1471-2407