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Table 1 Microarray analysis results from HepG2 and HepaRG® cells

From: microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line

miRNA Glucose (mg/L) Ratio mRNA Glucose (mg/L) Ratio
900 200 900 200
A-1) The 10 genes showing the great increase in HepG2 cells
2003miR-150-3p 35.2 185.4 5.27 IFI27 26 281 10.81
miR-575 97.3 471.9 4.85 HSPA1B 287 2009 7.00
miR-3158-5p 41.1 173.7 4.23 IF16 22 144 6.55
miR-593-5p 27.8 112.6 4.05 CMBL 53 296 5.58
miR-1273e 41.2 164.3 3.99 SULF2 19 106 5.58
miR-6724-5p 1080.5 4078.7 3.77 CYP1A1 25 135 5.40
miR-671-5p 71.8 265.8 3.70 CAPN3 54 286 5.30
miR-4725-3p 141.9 487.1 3.42 DDB2 125 624 4.99
miR-8059 154.7 520.5 3.36 NT5DC4 24 119 4.96
miR-373-5p 44.5 144.6 3.25 LY96 160 792 4.95
A-2) The 10 genes showing the great decrease in HepG2 cells
miR-106a-5p 2174.7 1259.1 0.58 KIF21A 338 38 0.11
miR-15b-5p 243.3 148.8 0.61 SLCC7A11 273 33 0.12
miR-93-5p 684.5 421.1 0.62 ANKRD12 354 48 0.14
miR-20a-5p 1793.2 1219.7 0.68 GOLGA4 249 36 0.14
miR-17-5p 2144.4 1479.7 0.69 TNKS2 104 15 0.14
miR-455-3p 213.3 147.6 0.69 MMD 232 34 0.15
miR-16-5p 1133.7 792.8 0.70 SLC38A2 1313 202 0.15
miR-19a-3p 381.5 274.8 0.72 ARHGAP18 356 55 0.15
miR-18a-5p 306.7 220.9 0.72 ZNF12 116 19 0.16
miR-26b-5p 966.8 775.9 0.78 ATP2B1 244 40 0.16
B-1) The 10 genes showing the great increase in HepaRG® cells
miR-6829-5p 44.8 102.3 2.28 RGS9 129 280 2.17
miR-3679-5p 164.8 225.4 1.37 SLC4A7 113 226 2.00
miR-4675 177.4 234.8 1.32 MY09A 56 112 2.00
miR-6726-5p 87.3 104.7 1.20 CAMSAP1L1 62 123 1.98
miR-6799-5p 203.8 238.3 1.17 SDCCAG1 101 197 1.95
miR-6803-5p 737.2 818.5 1.11 BCL9L 250 483 1.93
miR-6858-5p 181.7 197.9 1.09 PPHLN1 266 497 1.87
miR-6779-5p 148.0 148.0 1.07 BAT2L 350 652 1.86
miR-6721-5p 150.4 150.4 1.06 ZNF783 77 142 1.84
miR-6819-5p 198.4 198.4 1.05 RRAD 74 136 1.84
B-2) The 10 genes showing the great decrease in HepaRG® cells
miR-26b-5p 205.5 85.9 0.42 ESD 252 124 0.49
miR-191-5p 102 44.5 0.44 HMGCS1 4950 2531 0.51
let-7 g-5p 212.4 101.1 0.48 DDIT3 205 109 0.53
miR-19b-3p 290.3 138.3 0.48 MAP3K4 107 57 0.53
miR-20b-5p 103.1 50.0 0.48 GPR125 121 66 0.55
miR-1246 4912.0 2388.6 0.49 SMAD1 101 55 0.54
let-7e-5p 215.6 107.8 0.50 WBSCR1 305 167 0.55
miR-100-5p 208.6 104.3 0.50 PGM3 132 72 0.55
miR-148a-3p 256.5 130.0 0.51 THRSP 442 253 0.57
miR-122-3p 215.8 112.9 0.52 PSMB6 1013 600 0.59
  1. The result of microarray analysis of differently expressed miRNAs and mRNAs when HepG2 and HepaRG® cells were cultured in different glucose concentrations. Genes whose expression was greatly altered after the incubation of cells with two different concentrations of glucose, 900 mg/dL and 200 mg/dL, are shown. The 10 genes showing the great increase (A-1, B-1) and the 10 genes showing the greatest decrease (A-2, B-2) from 900 mg/L to 200 mg/L glucose are shown. The difference in the gene expression in HepG2 cells is shown in Table 1A, whereas that of HepaRG cells® is shown in Table 1B