Fig. 5
From: microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line
Caspase-3 activity induced by hydrogen peroxide in cells after incubation with various concentrations of glucose. Cells were cultured with 200, 900, and 1800 mg/L of glucose for 1 week and then 0.8 mM of hydrogen peroxide was added to the culture. After incubation for 24 h, cells were collected and caspase-3 activity was measured with absorbance at 405 nm. The ratios of the absorbance with and without H2O2 were calculated. Error bars indicate the standard error (SE) of four different experiments, as calculated from the data of the calculated ratios. ***p < 0.001 (n = 4 each)