Fig. 3
From: Iterative sorting reveals CD133+ and CD133- melanoma cells as phenotypically distinct populations
CD133+ and CD133- cells are equally tumourigenic with distinct phenotypes. a Serial xeno-transplantation of CD133+ and CD133- cells over 7 generations (Gen0 to Gen6) in NOD/SCID mice. Serially sorted cells from 3 primary cell lines were injected sub-cutaneously (3–5 mice per group) and tumours grown to ~200 mm3. Tumours were pooled, resuspended and cultured for ~2 weeks before reinjection. Data representative of 2 replicate experiments. b Flow cytometry of CD133 surface expression of CD133+ and CD133- cells prior to injection into NOD/SCID mice, over 7 generations (Gen0–Gen6). Data representative of 2 replicate experiments