Fig. 1

CD133+ and CD133- are not stem-like but display distinct phenotypes. a CD133 cell surface expression quantified by flow cytometry in 3 human primary lines derived from Stage IV melanoma metastases (LM-MEL-15, LM-MEL-34, LM-MEL-62). CD133 mRNA expression measured by real-time RT-PCR. Average of 3 replicate experiments +/- standard error. b Gene-expression profile for CD133+ (right bars, green) and CD133- (left bars, red) from 7 human primary malignant melanoma lines, using Gene Set Enrichment Analyses (GSEA) with a 5 % False Discovery Rate cut-off. Selected gene sets shown, with gene set size given in parentheses. Shading shows percentage of genes contributing to Enrichment Score: hatching, 20–50 %; grey, 50–75 %; black, 75–100 %. Nominal P values: *, P < 0.05, **, P <0.01, ***, P < 0.001. c Distribution pattern of CD133+ (green) and CD133- (red) microarray gene expression profiles compared to human Embryonic Stem (hES), induced Pluripotency Stem (iPS) cells (black), and fibroblasts and melanocytes (blue). Grey depicts global distribution of gene arrays in the Gene Expression Omnibus (GEO) database