Fig. 5
From: 53BP1 depletion causes PARP inhibitor resistance in ATM-deficient breast cancer cells
KU55933 reduced Olaparib induced ATM-Chk2 pathway activation and Rad51 up-regulation while knockdown 53BP1 supressed these effects. a, Western blot assay showed the protein level of Rad51, Chk2, phospho-Chk2 (Thr68), ATM and phosphor-ATM (Ser1981) in CAL-51 and MCF-7 cells after treatment with 10 μM Olaparib, 10 μM KU55933 and their combination respectively for 48 h. b, Western Blot assay showed the protein level of Rad51, Chk2, phospho-Chk2 (Thr68), ATM and phosphor-ATM (Ser1981) in CAL-51-sh53BP1, MCF-7-sh53BP1 cells and their control-transfected cells after synergistic treatment with 10 μM Olaparib and 10 μM KU55933. All experiments were performed at least three times