Fig. 3

IL-1β increased the invasiveness of luminal-type breast cancer cells in a TG2-dependent manner. a-b MCF7_Cont and MCF7_TG2 cells were allowed to invade through Matrigel for 48 h in the presence or absence of IL-1β (10 ng/ml). a Invaded MCF7_Cont and MCF7_TG2 cells (crystal violet). b Invaded cells were counted using ImageJ software. c-d MCF7_TG2 cells were allowed to invade through Matrigel for 48 h in the presence of IL-1β (10 ng/ml) and anti-IL-6 antibody (10 μg/ml). c Invaded MCF7_TG2 cells (crystal violet). d Invaded cells were counted using ImageJ software. Data are presented as mean ± SD based on three independent experiments using samples from triplicate cell cultures