Fig. 4

Real-time cell migration (a) and proliferation (b) analysis of SEC62-overexpressing and Sec62-depleted HeLa cells. a The cell index was measured as an indicator for migration 15 h after seeding identically pretreated HeLa cells and compared with the respective control cells. b The slope of cell proliferation curve was measured during the phase of exponential growth (50–74 h after seeding the cells) for HeLa cells transfected with SEC62 siRNA or a SEC62 plasmid and compared with cells transfected with control siRNA or a control plasmid. The experiments were repeated fourfold (n = 4) and a triplicate of every cell population was analyzed in each experiment. Cell migration (a) and cell proliferation (b) are presented as a percentage of the respective controI cells (=100 %) using box and whisker blots. Each box represents the range from the first quartile to the third quartile. The median is indicated by a line. The whiskers outside the boxes represent the ranges from the minimum to the maximum value of each group