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Fig. 3 | BMC Cancer

Fig. 3

From: High sensitivity isoelectric focusing to establish a signaling biomarker for the diagnosis of human colorectal cancer

Fig. 3

Detection of PLCγ1 total protein by isoelectric focusing. a. Immunoblotting of selected tissue samples with antibodies against PLCγ1. Blotting for GAPDH and β2 microglobulin (β2M) were used as loading control. b. Representative electropherogram showing PLCγ1 total protein peaks. Inset; electropherogram showing HSP70 run in parallel. c. Plot of PLCγ1 peak areas in samples from normal tissue, CRC stage II and IV biopsies. Values were normalized to HSP70 levels. Symbols in plots: Red; KRAS mutated, green; BRAF mutated, blue; wild type (WT) with regard to KRAS and BRAF, black; unclear for KRAS and BRAF

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BMC Cancer

ISSN: 1471-2407

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