Fig. 6

TBX3 overexpression in DCIS-like 21NT cells alters expression of key regulatory and EMT/invasion-associated genes. a Heat map (84 genes commonly dysregulated in breast cancer) showing absolute mRNA expression of 84 breast-cancer related genes within the 21NT transfectant cells. b Fold changes (mRNA) of genes significantly altered in expression in a similar fashion with overexpression of both TBX3iso1 and TBX3iso2 in 21NT cells. Data analysis was conducted by SA Biosciences PCR Array Data Analysis Web portal using the ΔΔCt method normalized to acidic ribosomal phosphoprotein P0 (RPLP0) expression. Fold changes are compared to 21NT + EV control and are organized based on increasing fold change of TBX3iso1. The 24 genes shown had similar alterations in gene expression with statistically significant fold changes for both isoforms (* p < 0.05, ** p < 0.01, ***p < 0.001). Genes in green had reduced expression, and genes in red had increased expression at the mRNA level with TBX3 isoform overexpression. Results are representative of 3 RT² PCR arrays per cell line. c Graphical representation of fold changes in panel (a). Dotted line represents normalized expression of transcript levels of empty vector control cells (fold change = 1). d Gene expression changes with TBX3 isoform overexpression organized based on gene function groupings. Genes in green had reduced expression, and genes in red had increased expression at the mRNA level with TBX3 isoform overexpression. e Western blot showing increased expression of Vimentin, Twist and Src protein levels with TBX3 overexpression