Fig. 4

The detection of 4T1-derived metastatic colonies in the bone marrow is increased in Lgals3−/− mice. Representative immunohistochemical staining of hematoxylin and eosin in the bone marrow of Balb/c a Lgals3+/+ or b Lgals3−/− mice. Representative immunohistochemical staining of CK-19 in the bone marrow of Balb/c c Lgals3+/+ or d Lgals3−/− mice previously inoculated with 105 4T1 mammary carcinoma cells in the fourth mammary fat pad for 28 days. e Number and representative images of clonogenic 4T1 metastatic cells cultured from a total of 105 iliac bone cells 21 and 28 days p.o.i. f CK-19 mRNA levels in iliac bone cells of Balb/c Lgals3+/+ or Lgals3−/− mice 15 days p.o.i. with 4T1 mammary carcinoma cells. Data are the mean ± S.D., n = 3, three animals per group; *p < 0.05, **p < 0.01