Fig. 6

CHEK2 upregulation represses breast stromal fibroblasts and their paracrine procarcinogenic effects. CAF-169 cells transfected with CHEK2-ORF (CAF-169-CHEK2-ORF) or control plasmid (CAF-169-control). a Whole cell lysates were prepared from the indicated cells and were used for immunoblotting analysis. The numbers below the bands indicate the corresponding expression levels after loading correction against GAPDH. b Assessment of the migration/invasion abilities of CAF-169 cells expressing either CHEK2 ORF or control plasmid as described in Fig. 3. c SFCM were collected after 24 h of incubation from CAF-169-CHEK2-ORF cells (SFCM-CHEK2-ORF) and control cells (SFCM-control), and then were added to MDA-MB-231 cells. The migration and invasion abilities were assessed as described in Fig. 5a. Error bars represent means ± S.D. *: p value <0.001