Fig. 5
CHEK2-deficient fibroblast secretions stimulate invasion/migration in an SDF1- and IL-6-dependent manner and enhance mesenchymal features in breast cancer cells. a SFCM were collected after 24 h of incubation from TCF-169-CHEK2-siRNA and their control cells, and were added to MDA-MB-231 cells (105) that were seeded onto the upper compartment of the plates and incubated for 24 h. The numbers of invaded and migrated cells were presented in histograms. Error bars represent means ± S.D. *: p value <0.001, as compared to the control lane 1. b MDA-MB-231 cells were treated as indicated, and then the migration and invasion abilities were assessed as described in A. c MDA-MB-231 cells were treated with SFCM from the indicated cells for 24 h, and then cell lysates were prepared and used for immunoblotting analysis using the indicated antibodies