Fig. 6

Exogenous Palmitate decreases HER2 and HER3 expression in HER2/neu-positive SKBR3 cells. a SKBR3 cells were treated with 250 μM palmitate or vehicle for 24 h. C16 = palmitate. Total protein was harvested and subjected to western blot analysis to evaluate the protein expression changes for the HER-family proteins HER2, phospho-HER2 (p-HER2, Tyr1221/1222), HER3, phospho-HER3 (pHER3, Tyr1289)), EGFR and phospho-EGFR (pEGFR,Tyr1068). Each experiment was performed in triplicate, representative images are shown. b Quantification of the HER2, pHER2, HER3 and pHER3 western blots using ImageJ. c Intensities were normalized to loading control and vehicle-control. The data is presented as mean · SD. * = p · 0.05, ** = p · 0.01, *** = p · 0.001. Student’s t-test. n = 3