Fig. 2

Exogenous palmitate induces a distinct transcriptional response in HER2/neu-positive SKBR3 breast cancer cells. HER2/neu-positive SKBR3 and HER2-normal MCF7 breast cancer cells were treated with 250 μM palmitate or vehicle for 24 h. C16 = palmitate. Total RNA was harvested and subjected to microarray analysis using Affymetrix Gene ST 1.0 arrays. a Heatmap showing log2-ratios of altered transcripts between vehicle and palmitate treated samples (1.5-fold cut-off). b Venn diagram showing the number of genes that are up- or downregulated with palmitate treatment in SKBR3 and MCF7 cells (1.5-fold cut-off). c Results of the Functional Annotation Clustering analysis after GO-term enrichment of upregulated genes using the DAVID Bioinformatics Resource