Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors

Table 3 Apoptosis associated proteins change in group BF-rTK + GCV.

Gene	BF/GCV	BF-rTK/GCV	Ratio
Bad	100.03	895.52	8.95
Bax	100.15	1155.50^a	11.54
Bid	700.90	3450.87	4.92
Bim	100.38	1292.41	12.88
Bcl-2	100.25	1093.11 ^a	10.90
Bcl-w	100.5	1552.42	15.45
XIAP	463.48	100.25	0.22
Survivin	1047.24	296.30	0.28
Livin	100.38	605.98	6.04
Caspase 3	100.88	1651.20	16.37
Caspase 8	100.57	1256.01	12.49
Cytochrome C	100.35	1004.71	10.01
HtrA2	100.76	1578.36	15.67
c-IAP2	229.05	779.93	3.41
Fas	100.87	789.76	7.83
FasL	100.35	316.53	3.15
TNF-beta	568.88	1408.11	2.48
TRAILR-1	277.23	1306.83	4.71
TRAILR-2	138.21	1055.52	7.64
TRAILR-3	100.35	411.89	4.11
IGF-1	100.32	723.87	7.22
IGF-2	100.77	219.51	2.18
IGFBP-3	142.73	1173.35	8.22
IGFBP-4	96.06	857.88	8.93
IGFBP-5	100.32	760.31	7.58
IGFBP-6	100.33	741.30	7.39
Hsp60	100.35	2263.01	22.56
Hsp70	100.89	2325.38	23.05
Hsp27	100.38	1009.85	10.06
P21	472.51	1305.01	2.76

The boldface letters were anti-apoptosis proteins and the others were apoptosis proteins
The BF value was independent of Table 2 and they are read from two different films
^aThe ratio of Bax/Bcl-2 is 1.06
Thirty differential proteins were up-regulated more than 2-fold in group BF-rTK + GCV compared with the BF + GCV intratumor-treated group. To summarize, twenty-three pro-apoptosis associated proteins were increased from 2.48-fold (TNF-β) to more than 23.05-fold (Hsp70). The total of pro-/anti-apoptosis ratio was 5.20-fold and the ratio of Bax/Bcl-2 was 1.06 in group BF-rTK + GCV. The results indicated that BF-rTK + GCV induced the increased expression of many pro-apoptosis associated proteins

ISSN: 1471-2407