Fig. 4
Ex vivo kinase substrate phosphorylation. The color map visualizes normalized log2-transformed signal intensities from kinase substrate arrays incubated with lysates from the three cell lines treated for 24 h with ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), AZD8055 (mTOR inhibitor; 10 μM), or combo-Rx (10 μM of both compounds in combination). As quantified and categorized by the color codes, red corresponds to higher and blue to lower substrate phosphorylation levels relative to levels from the corresponding control cells (vehicle-treated). Substrates associated with PI3K-AKT and/or MAPK signaling pathways were retrieved from PathCards (http://pathcards.genecards.org/), applying the super-pathway definitions ‘PI3K-AKT signaling pathway’ and ‘MAPK signaling pathway’. The identity of each peptide substrate, vertically in order from top to bottom, is given in Additional file 4: Table S4