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Fig. 7 | BMC Cancer

Fig. 7

From: Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells

Fig. 7

SSd triggered BAX and BAK translocation, and reduced the mitochondrial membrane potential. (a) HSC-T6 cells were treated with SSd (1 μM) for 0, 15, 30 and 60 min. The purity of mitochondrial fraction was validated by Western blotting with specific antibodies of mitochondria marker COX3 and cytosolic marker GAPDH. (b) SSd increased BAK and BAX expression in the mitochondrial fraction. (c) Cytosolic proteins were also applied to Western blotting. COX3 and GAPDH were also detected to validate the purity of the cytosolic fraction. (d) SSd reduced BAK and BAX expression in the cytosolic fraction. (e) The mitochondrial membrane potential (∆ψm) was monitored using a MitoProbe JC-1 assay kit, and was analyzed by flow cytometry. (f) HSC-T6 cells were grown in 24-well chamber cover glasses; treated with 1 μM SSd for 0, 15, 30 and 60 min, and analyzed using a confocal laser scanning microscope. Mitochondria were stained by the mitochondria-specific probe MitoTracker® Deep Red FM (100nM)

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