Fig. 4

HSP70-2 gene silencing retards cellular growth, cell viability, colony forming ability of CRC cells. a Quantitative PCR shows significantly reduced expression of HSP70-2 mRNA in COLO205 and HCT116 cells when transfected with HSP70-2 shRNA3 and shRNA4 as compared to control NC shRNA or HSP70-2 shRNA1, 2. b HSP70-2 shRNA3 & shRNA4 transfected COLO205 and HCT116 cells show significant HSP70-2 protein ablation as compared to control NC shRNA or shRNA1, 2. β-actin was used as a loading control. c Cellular proliferation assay demonstrates the significant reduced cellular growth of COLO205 and HCT116 transfected with HSP70-2 shRNA3 and shRNA4 cells at 24 h, 48 h and 72 h. d Cell viability as analyzed by MTT assay depicts the significant reduction of viable cells when transfected with HSP70-2 shRNA3 and shRNA4. e Colony forming ability was significantly reduced when COLO205 and HCT116 cells were transfected with HSP70-2 shRNA3 and shRNA4. f Representative images of the colony forming ability of HSP70-2 shRNA3 and shRNA4 transfected COLO205 and HCT116 cells as compared to control NC transfected cells. *p < 0.0001, statistically significant. All the results are an average of triplicates (n = 3) and the experiments were repeated twice