Fig. 4

Effects of test compounds on cell death induction. a Non-small cell lung cancer (NSCLC) cells cultured in the presence of test compounds (C1–C5) at 10 μM for 72 h were harvested, and then cell cycle profiles were analyzed. Sub-G1 fractions in cells are presented as mean ± SE of 3 independent experiments. b NSCLC cells were cultured in the presence of test compounds (C1–C5) at 10 μM for 72 h (A549, LU99, and H1299 cells) or for 96 h (EBC-1 cells). Cells were harvested, and then annexin V/propidium iodide (PI) staining was performed. Representative histograms of three different experiments are shown, and the inset numbers are the percentage of annexin(+)/PI(−) and annexin(+)/PI(+) cells