Fig. 2

Proliferation, anchorage-independent growth and motility of bladder tumor cells following PIK3CA knockdown. Analysis of phenotypic consequences of PIK3CA knockdown. Bladder tumor cells with PIK3CA knockdown by shRNAs KD1 and KD2, non-specific control shRNA (NS) and vector control cells (C). a. Proliferation analysis. Cells were plated at 3x10⁴ cells per well in triplicate and counted on the days shown. Y axis is total cell number x 10⁶. Values represent mean ± S.E. Results are representative of triplicate experiments. b. Number of viable BFTC909 colonies with a diameter >50 μm in an area of 2.5 mm² after 3 weeks of incubation in soft agarose. c. Mean fluorescence of migrated DAPI-stained VM-CUB-3 cells migrating through a Transwell membrane towards a chemoattractant (serum-supplemented medium). Values represent mean ± S.E. Results are representative of triplicate experiments. * indicates statistical significant difference (ANOVA test). Values represent mean ± S.E