Fig. 5

Validation of interactions between CELF1 and TUG1. a Relative expressions of CELF1 in the mock, shRNA-A2, or shRNA-B2 transfected H520 cells were detected through qRT-PCR in three independent experiments. Significant CELF1 upregulation was noted in the TUG1 knockdown H520 cells. (*p < 0.05) (b) RIP experiments were performed on H520 cells by using EED, EZH2, and IgG. Coprecipitated RNA was tested through qRT-PCR for TUG1. c Six primer sets were designed for ChIP experiments on EZH2 and EED of the promotor regions of CELF1. qPCR was used to determine the quantitation of ChIP assays. Representative images of the three independent experiments are shown