Fig. 4
From: MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells
RAB35/MICAL1 regulates ROS generation. a Effects of RAB35 and MICAL1 on H2O2 generation. MCF-7 cells transfected with siRAB35 or siMICAL1 were in serum-free media overnight. Representative micrographs of those cells incubated with EGF (10 ng/mL for 30 min) and stained with CM2-DCFHDA. *: P < 0.05 in the cultures with EGF relative to the cultures without EGF. #:P < 0.05 in the cultures with EGF plus siRAB35 or siMICAL1 relative to the cultures with EGF. Scale bar, 100 μm. b Effects of RAB35 and MICAL1 on O2− generation in MCF-7 cells. *: P < 0.05 in the cultures transfected with siRAB35 or siMICAL1 relative to the cultures with control siRNA. c Quantification of O2− levels in the cells transfected with HA–MICAL1, HA tagged MICAL1 FAD-LIM domain and CC domain. *: P < 0.05 in the cells transfected with HA–MICAL1 or HA–FAD-LIM relative to cells transfected with vector. #: P < 0.05 in the cells transfected with CC domain relative to cells transfected with HA–MICAL1 or HA–FAD-LIM domain