Fig. 4
Assessment of V-ATPase cell surface expression using flow cytometry analysis. For each cell line, a ΔMFI was calculated as the difference between the mean fluorescence intensity (MFI) of ATP6V0A1 staining compared to unstained control, (MFIATP6V0A1 – MFIcontrol). The relative ATP6V0A1 expression was calculated by scaling all data to the cell line with the lowest ΔMFI for #PA525030 (UPN2). Error bars indicate SEM of three technical replicates