Fig. 5
From: Bile acids destabilise HIF-1α and promote anti-tumour phenotypes in cancer cell models
Dihydroxylated BAs impair invasion and migration of DU-145 cells. For invasion and migration assays, all cells were grown under hypoxic conditions (DMOG 200 μM), except for untreated cells. Boyden chambers alone (migration) and those containing Matrigel (invasion) were hydrated for at least 2 h with 500 μl serum free media in the bottom of the well and 500 μl serum free media in the chamber. Once hydrated, the media in the bottom was replaced with media supplemented with 10 % FCS and the media in the chamber was replaced with cells in media supplemented with 10 % FCS. After growing for 24 h at 5 % CO2 in a humidified atmosphere at 37 °C, cells were fixed, rinsed, dried and counted. Cell migration and invasion data were derived from three independent experiments, N = 3, S.D; * P < 0.05; ** P < 0.01