Fig. 2
From: Bile acids destabilise HIF-1α and promote anti-tumour phenotypes in cancer cell models
Dihydroxylated bile acids CDCA and DCA are not cytotoxic towards A-549, MCF-7 and DU-145 cells. For cytotoxicity assays, all cells were grown under hypoxic conditions (DMOG 200 μM), except for untreated cells. Cells in the presence of 100 μM dihydroxylated BAs (CDCA and DCA) were grown for 16 h and assessed for lactate dehydrogenase (LDH) production. Cytotoxicity was expressed as the mean percentage standard deviation (SD) (n = 3) of the total LDH released from cells treated with 0.1 % Triton X-100, which was given an arbitrary value of 100 %. A-549 cells (a), MCF-7 cells (b) and DU-145 cells (c) exhibited minimal cytotoxicity in the presence of BAs. DU-145 morphology in the presence of BAs was assessed by phase contrast microscopy (d). Cells incubated with BAs exhibited cell shape and morphology similar to untreated cells. Data presented represent those from three independent replicates. Student T-tests were performed for comparison of treated cells with control untreated cells (* P < 0.05)