Fig. 3

Overexpression of EHD1 confers resistance to CDDP in NSCLC cells. a Western blotting analysis to confirm the overexpression of EHD1. GAPDH was used as a control to verify the equal loading of protein. Relative protein levels of EHD1 were determined. b Inhibitory effect of CDDP was measured in NSCLC cells using the CCK-8 assay at 24 h. c The IC50 values for CDDP were determined in NSCLC cells at 24 h. d Inhibitory effect of CDDP was measured at 48 h. e The IC50 values for CDDP were determined at 48 h. f Annexin-V-FITC/PI staining was analysed by flow cytometry in NSCLC cells after treatment with CDDP for 24 h. g The histogram demonstrates the percentage of early apoptotic NSCLC cells in response to CDDP concentrations of 0, 2.5, 5 and 10 μM