Fig. 1

Silibinin stimulated the generation of ROS derived from mitochondria in PC-3 cells. a PC-3 cells were treated with 150 μM silibinin up to 24 h and ROS production was determined by the fluorescence of DCFH-DA with flow cytometry. b PC-3 cells were treated with 150 μM silibinin for 12 h with the presence or absence of 0.5 μM DPI, 5 mM NAC, 10 μM Tempol and 100 U/ml CAT. c Representative images showed mitochondrial ROS production by confocal microscopy with MitoSOX, a mitochondrial ROS dye. Data are presented as ^* p <0.001 vs. the control group