Fig. 2

SOX2 induces a CSC state in CRC cells. Factors associated with a CSC state was evaluated in Caco2 cells and Caco2 cells stably transfected with SOX2 (Caco2-SOX2). a Proliferation of cells as measured by XTT cell proliferation assay. b Morphological evaluation of cells. c Expression of cellular adhesion molecules as evaluated by RT-PCR. Shown is relative expression with Caco2 cells set as 1. d Expression of cancer stem cell markers as evaluated by RT-PCR. Shown is relative expression with Caco2 cells set as 1. Gene expression analyses were reproduced three times and mean values ± SD is shown. Significant differences are indicated by * (P < 0.05)