Fig. 1

The nucleotide sequence design for the detection of the PIK3CA^H1047R mutation by PCR-RFLP. A 126-bp fragment covering PIK3CA exon-20 was chosen from the human genome for PCR amplification. Primer sequences are highlighted in blue. The forward primer sequence harbors one mismatched site (a → G, shown in red) to creating a new TGCGCA sequence for the FspI restriction endonuclease recognition site