Fig. 6

Everolimus enhances tamoxifen sensitivity. a MCF-7, MCF-7:5C and MCF-7:2A cells were seeded in single cell suspension and allowed to proliferate for 9 days in the presence of 20 nM everolimus, 1 μM 4-hydroxy tamoxifen (4OHT) or vehicle for 48 h. The plates were photographed and the number of colonies was quantified using Image J. Bar graphs represent the data from three independent experiments in triplicate and values are mean ± SD. *p < 0.05, **p < 0.01. *** p < 0.001. b Western blot indicates the effect of each treatment on mTOR phosphorylation and ERα, HSP90 expression and cleaved PARP