Fig. 3
Everolimus reduces anchorage-independent growth. a MCF-7, MCF-7:5C and MCF-7:2A cells were seeded in single cell suspension within a layer of 0.5 % agarose gel. Cells were treated with 20 nM everolimus or vehicle every 48 h for 17 days. Images were taken at 1X magnification and the number of colonies quantified using Image J. b Images of the same wells were taken at 10X magnification and colony diameter in μm2 is shown. Bar graphs represent the data from three independent experiments in duplicate. The displayed values are mean ± SD. ** p < 0.01