Fig. 4
Ly6Chi cells are required for 4 T1 tumor growth. BALB/c mice were inoculated with 105 4 T1 cells s.c. Gr1+ and Ly6G+ cells were depleted by the i.p. injection of 500 μg anti-Gr1 and anti-Ly6G antibody, respectively, one day prior to the inoculation of 4 T1 cells. Isotype control antibody 500 μg was injected into a control group of mice. Tasquinimod (25 mg/kg/day) was given in the drinking water from the day of inoculation and throughout the first week of tumor growth. Once palpable, tumors were frequently measured throughout the study and collected at day 14 post-inoculation. a, 4 T1 tumor growth curves (left) and tumor weights at day 14 post-inoculation (right) (ctrl, n = 7; tasq, n = 6; anti-Gr1, n = 7; anti-Gr1 + tasq, n = 6; anti-Ly6G, n = 7). Representative results of two independent experiments are shown. b, Number of splenic Ly6Chi (left) and Ly6Ghi (right) cells 1, 3 and 6 days after cell depletion using the indicated antibodies. Pooled data from two independent experiments are shown (n = 4–6 for all groups). c, Representative FACS plots showing the efficiency of cell depletion in day 1 mice shown in b. n.s. = not significant, *P < 0.05, **P < 0.01, Mann–Whitney U-test