Fig. 5
The role of Akt/HOTAIR signaling in Dp-induced cytotoxicity on breast cancer cells. a The ChIP-qPCR detections of IRF1 binding to the HOTAIR promoter in breast cancer MDA-MB-231 cells treated with Dp for 24 h. b Western blot detections of IRF1, p-Akt and Ake in Dp-treated MDA-MB-231 cells transfected with IRF1 siRNA (TCanti-IRF1). c Effects of Dp treatment for 24 h on the levels of HOTAIR in MDA-MB-231 cells and TCanti-IRF1 cells. d Effects of Dp treatment for 48 h on the cells viability in MDA-MB-231 cells and TCanti-IRF1 cells. The data are presented as the mean ± SD (n = 3). * P < 0.05 and ** P < 0.01 compared with MDA-MB-231 cells; # P < 0.05 and ## P < 0.01 compared with the control