Fig. 4

Effects of Dp treatment on Akt/IRF1 signaling pathway in breast carcinogenesis and breast cancer cells. a Western blot detections for the levels of p-Akt, Akt and IRF1 in CarT and CarT-Dp (40 μM Dp) cells in the cellular breast carcinogenesis model, as well as in breast cancer cells MDA-MB-231 and MCF-7 treated with Dp for 24 h. b Immunofluorescence analysis of IRF1 levels in breast cancer cells MDA-MB-231 treated with different concentrations of Dp for 24 h. Immunostaining density was quantified using Image J analysis. c The levels of Akt activity in MCF10A, CarT and CarT-Dp (40 μM Dp) cells in the cellular breast carcinogenesis model, as well as in breast cancer cells treated with Dp for 24 h. d Immunohistochemistry detections of p-Akt and IRF1 expression in breast tumors in athymic mice. Immunostaining density was quantified using Image J analysis. The data are presented as the means ± SD (n = 3). ^* P < 0.05 and ^** P < 0.01 compared with MCF10A cells; ^# P < 0.05 and ^## P < 0.01 compared with CarT cells or the control