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Table 4 FISH spots detected per CTC in a patient with an ALK-rearranged tumor depending on the step

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID Number of FA-FISH spots
  0.5 μm 0.6 μm 0.7 μm 0.8 μm
1 16 16 16 16
2 16 16 16 16
3 32 32 32 28
4 32 32 28 27
5 16 16 14 13
6 32 32 32 32
7 14 14 14 14
8 13 16 12 16
9 29 28 32 32
10 16 16 16 16
11 16 16 16 16
12 28 26 31 30
13 32 32 32 30
14 32 32 32 32
15 14 14 14 14
16 27 28 18 27
17 16 16 16 16
18 14 14 14 12
19 30 32 30 27
20 16 16 16 14
21 16 16 16 16
22 32 32 31 22
23 32 32 32 27
24 32 32 32 32
%a 79 % 92 % 75 % 58 %
  1. Abbreviations: ALK anaplastic lymphoma kinase gene; CTC, circulating tumor cell; FISH, fluorescence in situ hybridization
  2. aPercentage of cases where a higher number of FISH spots were observed
  3. The numbers in bold correspond to the highest number of spots for this CTC