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Table 1 FISH spots detected per CTC in a patient with an ALK-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID

Number of FISH spots

 

5 z-stacks

10 z-stacks

15 z-stacks

20 z-stacks

25 z-stacks

30 z-stacks

1

n.ia

9

9

13

10

18

2

3

8

6

16

15

16

3

n.ia

3

2

8

7

12

4

4

11

2

4

13

13

5

n.ia

5

6

8

8

17

6

n.ia

12

4

4

2

16

7

8

6

8

19

1

27

8

2

4

4

8

10

15

9

2

2

3

13

7

16

10

n.ia

22

6

14

17

27

11

n.ia

4

8

8

10

16

12

1

10

6

16

12

16

13

1

4

2

16

2

3

14

n.ia

6

9

14

14

14

15

2

4

n.ia

4

4

16

16

1

10

8

2

7

16

17

n.ia

6

9

8

20

9

18

6

7

7

16

14

16

19

6

7

n.ia

10

12

18

20

3

8

6

6

8

18

21

1

3

9

6

8

8

22

2

5

6

n.i.a

14

8

%b

0 %

0 %

4 %

23 %

18 %

82 %

  1. Abbreviations: ALK anaplastic lymphoma kinase gene; CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC