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Table 1 FISH spots detected per CTC in a patient with an ALK-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID Number of FISH spots
  5 z-stacks 10 z-stacks 15 z-stacks 20 z-stacks 25 z-stacks 30 z-stacks
1 n.ia 9 9 13 10 18
2 3 8 6 16 15 16
3 n.ia 3 2 8 7 12
4 4 11 2 4 13 13
5 n.ia 5 6 8 8 17
6 n.ia 12 4 4 2 16
7 8 6 8 19 1 27
8 2 4 4 8 10 15
9 2 2 3 13 7 16
10 n.ia 22 6 14 17 27
11 n.ia 4 8 8 10 16
12 1 10 6 16 12 16
13 1 4 2 16 2 3
14 n.ia 6 9 14 14 14
15 2 4 n.ia 4 4 16
16 1 10 8 2 7 16
17 n.ia 6 9 8 20 9
18 6 7 7 16 14 16
19 6 7 n.ia 10 12 18
20 3 8 6 6 8 18
21 1 3 9 6 8 8
22 2 5 6 n.i.a 14 8
%b 0 % 0 % 4 % 23 % 18 % 82 %
  1. Abbreviations: ALK anaplastic lymphoma kinase gene; CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC